Composite

Part:BBa_K4156114:Design

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-07)


pLldR-mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 680
    Illegal AgeI site found at 1908
    Illegal AgeI site found at 2020
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pLldR-mRFP uses the classical red fluorescent protein ( BBa_E1010 ) and the terminator rrnB T1 terminator ( BBa_B0015 ) assembled with the team's optimized pLldR promoter ( BBa_K4156101 ).


Source

Experimental constructed.

References